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Pilot trial at Sierra Streams Institute tests eugenol and TMS as humane pre‑preservation anesthesia for benthic macroinvertebrate samples
Summary
Sierra Streams Institute piloted a field anesthesia step—using eugenol and TMS—to reduce stress in benthic macroinvertebrates prior to ethanol preservation; initial results show many taxa anesthetize rapidly and staff report reduced distress.
Sierra Streams Institute (SSI) staff presented a pilot study testing whether a short anesthesia step can reduce stress responses in benthic macroinvertebrates before preservation in ethanol.
Motivation and methods: SSI staff noted volunteer and staff concern about placing live invertebrates directly into ethanol. Drawing on published work showing reduced stress markers in some aquatic invertebrates after exposure to anesthetic agents, SSI tested two agents—eugenol (a clove‑oil derivative) and TMS/MS‑222—at field‑practical…
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